Establishment of a Stable Cell Line Expressing"Toxic" Transient Receptor Potential A1 Channel
- VernacularTitle:稳定表达"毒性"瞬时受体势A1通道细胞系的建立
- Author:
Shaopeng WANG
;
Lianghui MA
;
Jixian LI
;
Zhigang PENG
;
Jing CAO
- Publication Type:Journal Article
- Keywords:
transient receptor potential A1 (TRPAl);
stable cell line;
toxicity
- From:
Progress in Biochemistry and Biophysics
2008;35(12):1378-1386
- CountryChina
- Language:Chinese
-
Abstract:
Transient receptor potential A1 (TRPAl) is a cold sensitive cation channel, which could also be activated by various pungent compounds. As a transduction channel in a number of sensory modalities, TRPAl expressing in heterogonous systems serves to provide great convenience in pharmacological analysis and functional investigation. Due to cellular toxicity, establishment of stable TRPAl cell line has always been challenging. Nevertheless, the first stable human embryonic kidney (HEK-293) cell line with un-controlled expression of TRPAl was successfully established. It was also confirmed that this stable cell line retained TRPAl expression for more than 25 passages in culture. The functional analysis of the cell response verified the stability and specificity of this novel recombinant TRPAl cell line. Altogether, the data indicated this TRPAl-HEK cell line would be a useful tool for functional analysis of TRPAl and for the development of high throughput screening (HTS) compatible assay in the effort to identify TRPAl modulators.
