Inhibitory effect of paeonol on hydrogen peroxide-induced apoptosis in PC12 cells

VernacularTitle:丹皮酚对过氧化氢诱导的PC12细胞凋亡的抑制作用
Author: Daohua XU ; Chenhui ZHOU ; Tie WU ; Bilian XU
Publication Type:Journal Article
Keywords: paeonol; hydrogen peroxide; apoptosis; PC12 cells
From: Chinese Journal of Pharmacology and Toxicology 2008;22(6):401-405
CountryChina
Language:Chinese
Abstract: AIM To investigate the inhibitory effect of paeonol on hydrogen peroxide(H2O2)-induced apoptosis in PC12 cells. METHODS The injury model in PC12 cells was generated by H2O2 treatment. The cell viability was determined using methylthiazolyl tetrazolium reduction assay. Apoptotic cells and reactive oxygen species (ROS) were measured by flow cytometry. Lactate dehydrogenase (LDH) activity and malonyldialdehyde (MDA) content were measured by spectroscope respectively. RESULTS After PC12 cells were treated with H2O2 (100 μmol*L-1) for 10 h,its viability obviously decreased, and apoptotic cells, LDH release into the culture media, ROS and MDA contents in PC12 cells significantly increased. When the cells were pretreated with paeonol (12, 25 and 50 μmol*L-1)for 1 h prior to incubation with H2O2, its viability was greatly increased, and apoptotic cells, LDH release, ROS and MDA contents significantly decreased. CONCLUSION Paeonol protects PC12 cells from H2O2-induced apoptosis and this effect is probably achieved through its antioxidative action.