Determination of cocaine and benzoylecgonine in guinea pig's hair after a single dose administration by LC-MS/MS
- VernacularTitle:LC-MS/MS法测定豚鼠毛发中可卡因及其代谢物苯甲酰爱康宁
- Author:
Qiran SUN
;
Ping XIANG
;
Hui YAN
;
Min SHEN
- Publication Type:Journal Article
- Keywords:
cocaine;
benzoylecgonine;
LC-MS/MS;
single dose;
hair
- From:
Acta Pharmaceutica Sinica
2008;43(12):1217-1223
- CountryChina
- Language:Chinese
-
Abstract:
A sensitive LC-MS/MS method to determine cocaine and its major metabolite benzoylecgonine in guinea pig's hair has been established. About 20 mg of decontaminated hair sample was hydrolyzed with 0.1 mol·L-1 HCl at 50 ℃ overnight, in the presence of cocaine-d3 and benzoylecgonine-d8 used as internal standards, and then extracted with dichlormethane. The analysis was performed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Positive electrospray ionization (ESI+) and multiple reactions monitoring (MRM) mode were used. The limit of detection (LOD) for cocaine and benzoylecgonine was 1 pg·mg-1. The calibration curves of extracted standards were linear over the range from 5 pg·mg-1 to 250 pg·mg-1 (r2≥0.999 7). The method was validated and applied to the analysis of guinea pig's hair after a single dose administration of cocaine hydrochloride. Cocaine and benzoylecgonine were not only detected, but also quantified in guinea pigs hair.
