Allograft renal transplantation improves chronic renal failure verified by the quality and quantity of erythrocytes
- VernacularTitle:同种肾组织移植改善慢性肾功能衰竭贫血:红细胞质量和数量验证
- Author:
Dongwen WANG
;
Rongyao LIU
;
Feng RU
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2008;12(53):10591-10595
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND: Repeated blood transfusion and recombinant human erythropoietin (rhEpo) have been previously used to improve anemia following chronic renal failure; however, their clinical applications are extremely limited by various side effects. OBJECTIVE: To investigate the therapeutic effect of allograft renal transplantation with rhEpo on anemia following chronic renal failure via verieying quality and quantity of some examines. DESIGN, TIME AND SETTING: A randomized grouping animal study was performed in Laboratory of Physiology. Shanxi Medical University from June 2004 to March 2005.MATERIALS: Eighty healthy adult male Wistar rats collected as receptors were injected with doxorubicin hydrochloride (6.5 mg/kg)into caudal vein three times per week for six weeks in total. Right kidney was then exsected to establish model of chronic renal failure. Another 20 newborn Wistar rats (3-4 days old, of both Sexes) were selected as donor. RhEpo (2000 U/mL) was provided by Shangdong Ahua Pharmaceutical Company (batch number-99435). METHODS: 20 adult rats in the control group did not undergo any treatments, and then other 60 adult rats were randomly divided into three groups of 20 rats per group after chronic renal failure modeling. Adult rats in the transplantation group underwent multidrop wansplantation of renal tissue into renal envelop; adult rats in the rhEpo group were intraperitoneally injected with rhEpo (30U/kg) three times Der week for six successive weeks in total; adult rats in the model group did not undergo any treatrnents. MAIN OUTCOME MEASURES: SABC assay was used to detect expression and distribution of rhEpo in reDal tissue and graft. ELISA was used to directly measure level of serum rhEpo in angular vein, routine assay was used to measure level of hemoglobin, and the corresponding kits were used to measure content of Na+-K+ATPaSe. activity of superoxide dismutase (SOD).and level of malondialdehyde (MDA)in erythrocyte membrane. RESULTS: rhEpo-antigen positive reaction showed a strongly positive expression in the control group. a weakly positive or an absent expression in the model group, and a strongly positive expression in the transplantation group. There were significant differences in the model group as compared to other two groups(P＜0.01).After 30,45,and 60 days, rhEpo level and numbers of hemoglobin and erythrocytes were higher in the rhEpo group and transplantation group than those in the model group(P＜0.05-0.01).After 60 days,rhEpo level in the rhEpo group was higher than that in the transplantation group (P＜0.05).On the other hand,after 30,45,and 60 days,MDA content in the rhEpo group and transplantation group was lower than that in the model group(P＜0.05).but there were no significant difeerences between rhEpo group and transplantation group(P＞0.05).Changes of SOD and Na+-K+ATPase were opposite to MDA among these groups.CONCLUSION:Renal transplantation can increase quality and quantity of erythrocytes of rats with anemia caused by chronic renal failure.The eflfect is equivalent to rhEpo.
