Teratogenic effect of self-made silicon carbide ceramic by micronuclei test
- VernacularTitle:微核实验评价碳化硅的潜在致突变性
- Author:
Lizhu JIANG
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2008;12(49):9775-9778
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Safety of self-made silicon carbide ceramic(SiC)was evaluated before clinical application.OBJECTIVE:To study the biocornpatibility of low-Ni non-Be SiC alloy,detect its teratogenic effect to the mice with micronuclei test.DESIGN,TIME AND SETTING:Randomized controlled animal study was performed at Central Laboratory of China Medical Universitv from June tO December 2007.MATERIALS:86 mice were randomly divided into 8 groups by sex and body mass,including SiC(0.02,0.002,0.000 2 mL/kg)groups,Ni-Cr-Ni extract(0.02,0.002,0.000 2 mL/kg)groups,saline group,and 75 mg/kgcyclophosphamide group.with 10 mice in each group.METHODS:Mice in each group were administrated once and sacrificed after 24 hours.Bone marrow extracted from bilateral femur was used to make cell suspension.Slide specimens were fixed,dried,and dipped into Giemsa,The district which had complete cell,well-distributed,and well-coloration,was selected and counted under light mlcroscope,MAIN OUTCOME MEASURES:1000 micronucleated cells of polychromatic erythro-cytes(MNPCE)were counted,and ratio between polychromatic erythro-cytes(PCE)and normochromatic erythrocytes(NRBC),i.e.,P/N,wascalculated.RESULTS:Micronuclear rates in the cyclophosphamide group were higher than other groups(P＜0.01):however,there wete no significant differences in micronuclear rates among SiC ceramic group,Ni-Cr-Ni extract group,and saline group(P＞0.05);while,there were also no significant differences between SiC ceramic group and Ni-Cr-Ni extract grouP.The P/N value was≥1.CONCLUSION:The self-made SiC has no teratogenic effect to mice and shows great biocompatibility
