Fucosylated Glycans Associated With Development and Metastasis of Hepatocellular Carcinoma Cells
10.3724/SP.J.1206.2008.00291
- VernacularTitle:岩藻糖链与肝癌细胞发生和转移的相关性研究
- Author:
Lihui WU
;
Ping HU
;
Wei WU
;
Xingzhong WU
- Publication Type:Journal Article
- Keywords:
liver cancer;
Lewis antigens;
metastasis
- From:
Progress in Biochemistry and Biophysics
2009;36(1):33-41
- CountryChina
- Language:Chinese
-
Abstract:
Dynamic fucosylation of glycoprotein especially 80 ku which bound to UEA and LCA during the course of rat hepatocarcinogenesis was investigated. In patient hepatocellular carcinoma, more UEA- and LCA-bound proteins were also observed in patients with high metastatic potential than those with low metastatic potential. Fueosylated glycans constitute important adhesion molecules such as Lewis antigens. A differential expression pattern of Lewis antigens was further conf'Lrmed on various metastasis potential hepatocellular carcinoma cells (HCC). High metastatic hepatocellular carcinoma cell line (HMCC97H) expressed much more Lewis x and b than low metastasis HMCC97L cells. Moreover, surface Lewis x, or b expression level declined significantly after the cells were treated by retinoie acid. Not only in experimental metastasis foei, but in HCC as well, both α1,3/1,2 and α1,6 fucosyltransferase activities were quite high. After retinoie acid treatment, α1,3/1,2 fueosyltransferase activities were significantly inhibited, Lewis x on epidermal growth factor receptor reduced, and the EGFR was less phosphorylated. These results suggested that fucosylated glycans such as Lewis x played an important role in HCC development and metastasis.
