Effects of astragalus mongholicus and radix notoginseng on in vitro differentiation of bone marrow mesenchymal stem cells in patients with lower limb ischemia
- VernacularTitle:黄芪和三七对下肢缺血患者骨髓间充质干细胞体外分化的影响
- Author:
Bohua YANG
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2008;12(51):10189-10192
- CountryChina
- Language:Chinese
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Abstract:
BACKGROUND: It has been confirmed that Chinese medicine astragalus mongholicus and radix notoginseng can improve symptoms of lower limb ischemia. It is supposed that this kind of therapy is associated with the promotion effect of astragalus mongholicus and radix notoginseng on differentiation of bone marrow mesenchymal stem cells (BMSCs) into vascular endothelial cells.OBJECTIVE: To identify that whether astragalus mongholicus and radix notoginseng are effective in promoting the proliferation and differentiation of BMSCs into vascular endothelial cells. DESIGN, TIME AND SETTING: The in vitro differentiation experiment was performed at the Key Laboratory of Education Ministry, Dong Zhimen Hospital Affiliated to Beijing University of Chinese Medicine from April to June 2005. MATERIALS: Bone marrow blood from 23 patients with lower limb ischemia was collected routinely at the Dong Zhimen Hospital Affiliated to Beijing University of Chinese Medicine. The density gradient centrifugation was used to separate bone marrow mononuclear cells, I.e. BMSCs. Astragalus mongholicus injection (equal'to 2 g/mL crude drug, Diaojiuhong, China), Xuesaitongfen injection (main component radix notoginseng, Zhenbaodao, China), CD34 antibody (BD, USA) and FACS Calibur flow cytometer (BD, USA) were used in this study. METHODS: BMSCs were incubated with 3 mL low, middle and high doses of astragalus mongholieus (containing 4, 12 and 36 g/L crude drug), and 3 mL low, middle and high doses of Xuesaitong suspension (50, 100 and 200 mg/L radix notoginseng). BMSCs only cultured without drugs served as a blank control group. Medium was changed every other day, for successively 3 weeks.MAIN OUTCOME MEASURES: Three weeks later, cell morphology was observed under an inverted phase contrast microscope. CD34+ cell percentage was detected by flow cytometry.RESULTS: Three weeks after BMSC culture, most cells were spindle-shaped, fascicular, with shape and characteristics of endothelial cells. Compared with the blank control group, the percentage of CD34+ cells were significandy increased in the low and middle doses of astragalus mongholicus group and in the middle and high doses of radix notoginseng group (P < 0.05-0.01). CONCLUSION: Astragalus mongholicus and radix notoginseng have the function of promoting proliferation and differentiation of BMSCs into vascular endothelial cells in a dose dependent effect.
