Effect of disequilibrium of calcium homeostasis on apoptosis of MDCC MSB1 cells induced by lanthanum chloride
- VernacularTitle:钙稳态失衡在氯化镧诱导MDCC-MSB1细胞凋亡中的作用
- Author:
Lan ZHAO
;
Wenjun LI
;
Jintao WANG
;
Shiwen XU
- Publication Type:Journal Article
- Keywords:
lanthanum chloride;
MDCC-MSB1;
DNA damage;
alcium homeostasis;
apoptosis
- From:
Chinese Journal of Veterinary Science
2009;29(5):643-648
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the effect of the disequilibrium of calcium homeostasis on the apoptosis of MDCC-MSB1 cells induced by lanthanum chloride(LaCl3 ), MDCC-MSB1 cells was conventionally cultured in RPMI1640, which was tured for 24 h, MTT was utilized to detect the cell multiplication inhibition ratio,DNA ladder and TUNEL to detect apoptosis,the Fura-2/AM as the probe to detect the [Ca2+]i within cells. The results indicated when LaCl3 concen and [Ca2+]i also increased,and presented a dose-effectiveness relationship. LaCl3 could restrain the proliferation of MDCC-MSB1 cells,suggesting their apoptosis induced by changing [Ca2+]i.
