Guided tissue regeneration membrane, artificial bone, and basic fibroblast growth factors for treatment of periodontal bone defects
10.3969/j.issn.1673-8225.2009.12.019
- VernacularTitle:应用引导组织再生膜与人工骨、碱性成纤维细胞生长因子等生物材料治疗牙周骨缺损
- Author:
Xiaojuan QU
;
Jianhua ZHU
;
Tieyu LIU
;
Ying WANG
;
Weimin ZHANG
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2009;13(12):2291-2294
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND: Guided tissues regeneration (GTR) has been successfully used in the treatment of periodontal diseases by using biocompatible membrane to prevent tooth epithelial cell growing towards root and to facilitate the formation of a certain gap which is favorable for periodontal membrane cells to occupy the surface of the root, thereby to rebuild the peridental structure. OBJECTIVE: To verify whether GTR can promote the repair of periodontal bone defect and the formation of new bones and whether combined use of GTR membrane, artificial bone, and growth factors can acquire much more new periodontal tissue. DESIGN, TIME AND SETTING: An animal observation experiment was performed at the laboratory of Medical College of Dalian University between February and July 2006. MATERIALS: Basic fibroblast growth factor (bFGF) freeze-dry powder (50 mg/ampoule) was thoroughly dissolved with ddH2O to prepare 5 g/L bFGF solution, bFGF solution was dropped onto collagen membranes with a size of 10 mm ×10 mm (5 mg bFGFs per piece of collagen membrane). METHODS: Cementum and the alvelor bone 6 mm below the cement-enamel junction were removed to induce periodontal defect in 16 rabbits with big ears. Artificial bone with bFGF and GTR membrane were used to fold around the defect on the right side, followed by reduction and suture, serving as experimental side. The left side underwent identical experimental procedure, with the exception of application of artificial bone with bFGF and GTR membrane, serving as control side. MAIN OUTCOME MEASURES: Carrier material absorption, inflammation of inner connective tissues, and status of junctional epithelium were examined through the use of microscope. RESULTS: The control side exhibited no concrescence, while the expedmental side displayed concrescence of the cementum to various extents. CONCLUSION: Application of GTR membrane, artificial bone, and bFGF can promote the concrescence of damaging bone.
