Study on aminoglycoside resistance and drug resistance gene of ESBLs-producing Escherichia coil
10.3760/cma.j.issn.1673-4130.2009.03.005
- VernacularTitle:产超广谱β-内酰胺酶大肠埃希菌对氨基糖甙类药物耐药性及耐药基因研究
- Author:
Weiping ZHENG
;
Weifeng SHI
- Publication Type:Journal Article
- Keywords:
Escherichia;
Beta lactamases;
Drug resistance,bacterial;
Base sequence
- From:
International Journal of Laboratory Medicine
2009;30(3):220-222
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the characteristics of aminoglycoside resistance of extend-ed-spectrum β-lactamases(ESBLs)-producing Escherichia coli(E, cold and expression of aminoglyco-side-modifying enzyme genes. Methods The minimal inhibitory concentrations(MICs) of gentamicin,amikacin, kanamycin, tobramycin, netilmicin and neomycin for 37 strains of ESBLs-producing E. Coli were detected by agar dilution. In addition, six aminoglycoside-modifying enzyme genes were amplified by polymersae chain reaction(PCR) and verified by DNA sequencing. Results MICand MIC90 of gentamicin, amikacin, kanamycin, tobramycin and netilmicin for 37 strains of ESBLs-producing E. Co-Il all excelled 256 μg/mL, the resistance rates of the above antibiotics were 78.4%, 45.9%, 72.9%,83.8%and 64.90%, respectively. However, neomycin still had powerful antibacterial activity. In ad-dition, five modifying enzyme genes, including aac(3)-Ⅱ , aac(6′)-Ⅰ b, aac(6′)-Ⅱ , ant(2″)-Ⅰ and ant(3″)- Ⅰ genes, were found in 37 isoaltes except aac(3)- Ⅰ , and their positive rates were 56.8%,27.0 %, 2.7 %, 5.4 % and 13. 5 %, respectively. Conclusion The aminoglycoside resistance of ES-BLs-producing E. Coil may be associated with the expression of aminoglycoside-modifying enzyme genes.
