The reliability study of urinary microalbumin test by dry hemical method
10.3760/cma.j.issn.1673-4130.2009.03.008
- VernacularTitle:尿微量清蛋白干化学法测定的可靠性探讨
- Author:
Huabin XU
;
Juan YANG
- Publication Type:Journal Article
- Keywords:
Albuminuria;
Albumins;
Reproducibility of results
- From:
International Journal of Laboratory Medicine
2009;30(3):229-231
- CountryChina
- Language:Chinese
-
Abstract:
Objective The quantitation measurement of microalbuminuria was adopted to verify the feasibility of urinary microalbumin and ratio of mieroalbumin to creatinine (M/C) detection with dry hemieal method. Methods Totally 80 urine samples from the patients with clinically diagnosed dia-betes were tested by the immune quantitation method (immunoturhidimetric assay) and dry heroical method (HT2000 urine analyzer and test paper, Guilin Hnatong Company) simultaneously. Sarcosine oxidase method was applied to measuring creatinine level. Ten cases of outliers were removed. With the quantitation result of M/C as the reference standard, immune quatilyzation of the M/C as refer-ence, we compared and analyzed the results of the immune quantilization of urine microalbumin, those of the dry chemistry method and of the dry chemistry system detecting M/C. Results There was sig-nificant differences in test results of urinary microalbumin with the dry chemistry method and the im-mune quantitation method (P<0.01) The sensitivity, accuracy and Youden index of the microalbu-minuria testing had been observed to decrease in order in immune the dry chemistry method, the im-mune quantitation method and M/C quantitation detection method. Conclusion The sensitivity of semi-quantitation dry chemical method is satisfactory in detecting microalbumin, which may be used as a means of microalbumin screening. M/C detection with the dry chemical method isn't suitable for screening microalbumin in instant urine samples.
