Development and Identification of a Live Attenuated Influenza B Virus Vaccine Candidate
10.3724/SP.J.1206.2008.00542
- VernacularTitle:冷适应减毒B型流感病毒疫苗候选株的制备及鉴定
- Author:
Penghui YANG
;
Wenqi AN
;
Xinfu SHI
;
Yueqiang DUAN
;
Deyan LUO
;
Pengfei ZHANG
;
Chong TANG
;
Li XING
;
Yujing ZHANG
;
Xiufan LIU
;
Xiliang WANG
- Publication Type:Journal Article
- Keywords:
influenza B virus;
reverse genetics;
eight-plasmid system;
reassorted influenza virus
- From:
Progress in Biochemistry and Biophysics
2009;36(3):358-363
- CountryChina
- Language:Chinese
-
Abstract:
A cold-adapted (ca), temperature sensitive (ts), live attenuated influenza B virus strain B/Ann Arbor/1/66 was chosen for influenza virus rescue research, in which six internal gene segments, PB1, PB2, PA, NP, M, NS, were fully synthesized and nine amino acid substitutions were artificially alter by human intervention. The resultant B/Ann Arbor/1/66 plasmids were named as pAB121-PB1, pAB122-PB2, pAB123-PA, pAB124-HA, pAB125-NP, pAB126-NA, pAB127-M and pAB128-NS, respectively. A recombinant influenza A virus was previously generated entirely from cloned cDNA. An infectious recombinant influenza B virus was generated here, and designated as rMDV-B, by plasmid-based reverse genetics. The rMDV-B virus contained HA and NA genes from an epidemic influenza B vires strain B/Malaysia/2506/2004 in the background of internal genes derived from influenza B virus strain B/Ann Arbor/1/66. HA titer of rMDV-B in MDCK cells and embryonated chicken eggs ranged from 1 : 64 to 1 : 512. The results may allow an effective live influenza B vaccine to be produced from a single master strain, providing a model for the design of future live human influenza vaccines.
