Application of VP1 Protein to Develop Monoclonal Antibody against Foot-and-mouth Disease Virus Asial Type
10.1007/s12250-009-2986-z
- Author:
Tong LIN
;
Junzheng DU
;
Junjun SHAO
;
Guozheng CONG
;
Shuai SONG
;
Shandian GAO
;
Huiyun CHANG
- Publication Type:Journal Article
- Keywords:
Asial type FMDV;
VP1 protein;
Monoclonal antibody
- From:
Virologica Sinica
2009;24(3):215-220
- CountryChina
- Language:Chinese
-
Abstract:
In order to develop an anti-FMDV Asial type monoclonal antibody (mAb), BABL/c mice were immunized with recombinant FMDV VP1 protein. Three mAbs, 1B8, 5E1 and 5E2, were then further optimized. The result indicated that prepared anti-FMDV Asial mAbs had no cross-reactivity with Swine vesicular disease (SVD) and FMDV O, A and C type antigen. Their titers in abdomen liquor were l:5×106, l:2×106 and l:5×l06, respectively. 1B8 was found to be of IgGi subtype, 5E1 and 5E2 belonged to IgG2b subtype. In this study, the prepared mAbs are specific for detecting FMDV type Asial, and is potentially useful for pen-side diagnosis.
