Genetic Variation Analyses of nsp2 Gene of PRRSV in Ningxia Hui Autonomous Region of China
10.1007/s12250-009-3017-4
- Author:
Hong TIAN
;
Jingyan WU
;
Shuanghui YIN
;
Youjun SHANG
;
Ziping MAN
;
Na ZHAO
;
Ye JIN
;
Xiangtao LIU
- Publication Type:Journal Article
- Keywords:
PRRSV;
nsp2 gene;
Homology analysis;
PRRSV Ningxia isolates (NX strain)
- From:
Virologica Sinica
2009;24(3):221-226
- CountryChina
- Language:Chinese
-
Abstract:
To gain a better understanding of the genetic diversity and evolution of PRRSV in the Ningxia Hui Nationality Autonomous Region (Ningxia) of China, the nsp2 genes from a series of PRRSV strains collected from the region in 2007 were partially sequenced. These sequences were then analyzed along with the classical strain (ch-la) and two other epidemic strains SD (3) and SD2006. Comparison of the nucleotide sequence with ch-la indicated that nsp2 genes of seventeen Ningxia isolates (NX strain) have deletions of 87 nucleotides. Sequence analysis indicated that homology between the Ningxia strain and ch-la was 60.3%-79.9% in the nucleotide sequence, and homology between the NX strains and SD strains was 80.3%-98.8% in the nucleotide sequence. The nsp2 genes of the seventeen isolates had 74.9%-100% nucleotide sequence identities with each other. This study was undertaken to assess the regional variation of prevalent PRRSV and to establish a sequence database for PRRSV molecular epidemiological studies.
