RNA Silencing Suppressor p19 Regulates The Expressions of Cell Cycle Related Genes

VernacularTitle:RNA沉默抑制子p19调控细胞周期相关蛋白表达
Author: Li LIU ; Jian LI ; Yuping XU ; Wentao QIAO ; Qimin CHEN ; Yunqi GENG
Publication Type:Journal Article
Keywords: RNAi; siRNA; miRNA; p19; G2/M arrest; cell cycle; cyclin A1; cyclin A2
From: Progress in Biochemistry and Biophysics 2009;36(5):541-548
CountryChina
Language:Chinese
Abstract: Besides its function as a pathogenicity determinant, the Tombusvirus P19 also serves as a suppressor of RNA interference (RNAi) by sequestering intracellular small RNAs such as the small interfering RNAs (siRNAs) and microRNAs (miRNAs). However, the effect of P19 on mammalian cells has not been evaluated before. A human embryonic kidney 293 cell line that stably expressed p19 (HEK293-p19) was generated. Flow cytometric analysis revealed that over-expression of P19 caused a significant accumulation of G2/M phase cells. Cell proliferation assays demonstrated a reduced DNA replication and cell growth in HEK293-p19 cells. Moreover, p19 altered the expression profiles of a number of cell cycle regulators in HEK293 cells, such as upregulafion of cyclin A1, CDK2, CDK4, CDK6, p18, cyclin D2, p19INK4d and E2F1, and downregulation of p15, cyclin A2, cyclin B1 and cyclin E1. Thus, the data strongly indicate that p19 might influence multiple G2/M regulators to cause G2/M arrest.