Immunomodulatory effects of cryolytic lung cancer cells on bone marrow-derived dendritic cells
- VernacularTitle:冻融肺癌细胞对骨髓树突状细胞的免疫调节作用
- Author:
Hongwu WANG
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2009;13(18):3560-3564
- CountryChina
- Language:Chinese
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Abstract:
BACKGROUND: Cryoimmunology has been paid more attention in recent years, but the effect of cryoablation on cellular function has not been clearly understood, especially for the cellular apoptosis and dendritic cells.OBJECTIVE: To evaluate whether NCI-H446 lung cancer cells could activate bone marrow-derived dendritic cells (DCs) which stimulate lymphocytes to be cytotoxic lymphocytes (CTLs) to kill cancer cells?DESIGN, TIME AND SETTING: Cellular immunology experiment was performed at the Hematological Laboratory, Beijing Naval General Hospital, and Genetics Center of Military Medical Science Academy of the PLA between August 2002 and April 2003.MATERIALS: NCI-H446 cells were small lung cancer cells purchased from Shanghai Institute of Sciences.METHODS: Cultured lung cancer cells were cryo-treated and added to cultured DCs in the presence of naive lung cancer cells. Morphological changes of cancer cells were observed; mixed lymphocyte reaction (MLR) and antitumor activity of cytotoxic T lymphocyte (CTLs) were also evaluated.MAIN OUTCOME MEASURES: Morphology of DCs and cell surface immunophenotype changes; MLR of cultured cells and cell apoptosis.RESULTS: ① of 51 patients,18 were followed-up for 6-12 months,2 for 13-18 months,and 8 for 19-24 months.② A total of 49 (96.1%) out of 51 patients preserved the eyeballs and the visual acuity improved to different degrees in 48 (94.1%) patients.③ After surgery,fungal infection recurred in 6 eyes (11.6%),4 of which were controlled by antifungal medication and 2 was enucleated because of uncontrolled endophthalmitis.Graft rejection was found in 18 (35.3%) eyes, 13 of which recovered transparent by medication and 5 received secondary PKP.Graft ulceration was present in 4 (7.84%) eyes, 3 of which were cured and the remaining one was re-grafted because of severe endothelial cell loss. Secondary glaucoma appeared in 7(13.7%) eyes,and the intraocular pressure was controlled medically and surgically.Complicated cataract occurred in 6 (11.8%) eyes,3 of which underwent cataract extraction. Most complications were successfully controlled.In the final follow-up period,45 (88.2%) grafts were transparent.CONCLUSION: Cryo-treated lung cancer cells exhibited cell lysis and membrane collapse, which activate bone marrow-derived DCs to kill cancer cells specifically.
