IL-18 augments protective immunity of Sj23 plasmid DNA vaccine against Schistosoma japonicum in mice
- VernacularTitle:IL-18增强日本血吸虫DNA疫苗Sj23的免疫保护效果
- Author:
Jingtao MEN
;
Quan LIU
;
Limin SHANG
;
Jiaojiao LIN
;
Zhiqing FU
;
Yaojun SHI
;
Zhiping XIA
;
Xichen ZHANG
- Publication Type:Journal Article
- Keywords:
Schistosoma japonicum;
Sj23;
IL-18;
DNA vaccine;
mice
- From:
Chinese Journal of Veterinary Science
2009;29(5):610-614
- CountryChina
- Language:Chinese
-
Abstract:
Two recombinant plasmids pVAX/Sj23 and pVAX/mIL-18 containing Schistosoma japonicum 23 000 membrane protein (Sj23) and murine IL-18 were evaluated for their ability to induce immune responses and to protect against S. japonicum challenge in mice. All animals vaccinated with pVAX/Sj23 alone or plus pVAX/mIL-18 developed specific anti-SWAP (soluble worm antigen preparation) ELISA antibody and splenocyte proliferation response,and co-injection of pVAX/mIL-18 significantly increased the production of IFN-γ and IL-2 compared with pVAX/Sj23 alone, indicating that IL-18 enhances the Th1-dominant immune response. The challenge experiment showed that worm reduction rates in pVAX/Sj23 group compared with control group (pVAX1) was 26.5% and in the pVAX/Sj23 plus pVAX/mIL-18 group was 41.9% ,and the hepatic egg reduction rates were 42.7 and 49.6%,respectively. These results indicated that co-injection of an IL-18 plasmid with Sj23 DNA vaccine efficiently improves the protective effect against S. japonicum infection.
