Clinical value of combined detection of autoantibodies in diagnosis of rheumatoid arthritis
10.3760/cma.j.issn.1673-4130.2009.04.010
- VernacularTitle:自身抗体联合检测诊断类风湿关节炎的研究
- Author:
Xinfeng WANG
;
Ping FANG
- Publication Type:Journal Article
- Keywords:
Arthritis,rheumatoid;
Autoantibodies;
Antibodiea,antinuclear;
Clinical La-boratory Techniques
- From:
International Journal of Laboratory Medicine
2009;30(4):339-341
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the clinical value of combined detection of antiperinuclear fac-tor (APF) ,anti-RA keratin antibody (AKA) and anticyclic citrullinated peptide antibody (anti-CCP) in diagnosis and differential diagnosis of rheumatoid arthritis (RA). Methods Totally 119 RA pa-tients,101 non-RA rheumatisants and 39 healthy controls were enrolled in the study. Enzyme linked immunosorbent assay (ELISA) was applied to quantitately detecting anti-CCP antibody; immunofluo-rescence method was applied to quantitately determining AKA and APF. Then the quadruple tabular form was used to calculate the sensitivity and specificity. Results In RA group,the sensitivity of APF, AKA and anti-CCP antibody was 48.8% ,32.3% and 83.5% respectively; the specificity was 92. 2%, 93. 1% and 94.1 % respectively. Conclusion Three autoantibodies,including APF,AKA and anti-CCP antibody have high specificity in early diagnosis of RA. Combined detection of the three antibodies con-tributes to diagnosis and differential diagnosis of rheumatoid arthritis (RA).
