A study on the stability of control preparation of blood cells after unsealing
10.3760/cma.j.issn.1673-4130.2009.05.014
- VernacularTitle:血细胞质控品开封后的稳定性研究
- Author:
Huabin XU
;
Yuanyuan LI
- Publication Type:Journal Article
- Keywords:
Blood cells;
Quality control;
Blood preservation;
Research
- From:
International Journal of Laboratory Medicine
2009;30(5):456-458
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the stability of control preparation of blood cells after un-sealing. Methods One vial of red blood cell control preparation was aliquoted in fifteen round bottom centrifuge tube with covers stored at 2-8 "C. Aliquots were tested respectively in modes of whole blood and pre-dilution each day. The test results were filled in the quality control chart. The quality control i- tems included hemoglobin (Hb), mean cell volume (MCV), hematocrit (HCT), white blood cells (WBC) ,red blood cells (RBC) and platelets (PLT). The stability of the blood cell control preparation was also dynamically observed for 15 days with a non-aliquot standard preparation. Results There were no significant differences in the test results among the aliquots tested from day 1 to day 15. The PLT count was significantly increased for the non-aliquoted vial on day 7 (P<0.05). The coefficientvariation (CV) of test results for pre-dilution specimens was 1.3 to 5.5 times as that for whole blood specimens. Conclusion The aliquots of blood cell control materials has the potential to remain viable for at least 15 days under the temperature of 2 ℃-8℃. The whole blood dilution method has preferable reproducibility and accuracy.
