Research on clinical distribution and antimierobiai resistance to bacterial biofiim in urinary tract infec-tions
10.3760/cma.j.issn.1673-4130.2009.05.016
- VernacularTitle:尿路感染生物被膜菌临床分布及耐药性研究
- Author:
Binbin ZOU
;
Yong QI
;
Yong WU
- Publication Type:Journal Article
- Keywords:
Urinary tract infections;
Biofilms;
Drug resistance,bacterial
- From:
International Journal of Laboratory Medicine
2009;30(5):461-463
- CountryChina
- Language:Chinese
-
Abstract:
Objective To clarify the clinical distribution and antimicrobial resistance characteris-tic of bacterial biofilm during catheter-associated urinary tract infection,and to simulate biofilm "real state" in vivo. Methods Totally 120 patients with catheter-associated urinary tract infections (UTIs) were enrolled in the study. The urine specimens were collected for screening biofilm strains and the corresponding planktonic strains. The biofilm was detected with semi-quantitative detecting method. Antibiotics susceptibility test were performed on the biofilm bacteria to clarify the difference of drug resistance in common MH medium the between biofilm strains and the corresponding planktonic strains,as well as the difference of drug resistance of positive strains of the biofilm between Poloxamer medium and common MH medium. Results Totally 48 strains (48/120,40%) of biofilm bacteria were detected. The antibiotic susceptibility test of planktonic and biofilm bacteria in Mueller-Hinton agar showed no significant difference (P>0.05), while the antibiotic resistance of biofilm bacteria in Muel-ler-Hinton agar and Poloxamer hydrogel was statistically different (P<0.05),and the former was stronger. Conclusion The biofilm bacteria during the urinary tract infection were mainly Staphylococci and Enterococci. The antimicrobial resistance of planktonic and biofilm bacteria have no significant difference in vitro, h is speculated that Poloxamer media may simulate the real living environment of biofilm bacteria and display their "true" drug resistance.
