Expression Patterns of Nedd4 Isoforms and SGK1 in the Rat Cochlea
- VernacularTitle:大鼠耳蜗组织中Nedd4及SGK1的表达
- Author:
Shixun ZHONG
;
Zhaohua LIU
- Publication Type:Journal Article
- Keywords:
Endolymph;
Cochlea;
Serum and glucocorticoid- inducible kinase 1;
Neural precursor cell-expressed,developmentally downregulated gene
- From:
Journal of Audiology and Speech Pathology
2009;17(4):369-372
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the cellular localization of the neural precursor cell-expressed, developmentally downregulated isoforms(Nedd4), Nedd4- 1/2 and Nedd4- 2, and the serum glucocorticoid- inducible kinasel(SGK1) in various subregions of the rat cochlea. Methods The expression patterns of Nedd4-1/2, Nedd42 and SGK1 in the cochlea of rat were studied by immunohistochemistry with the specific polyclonal rabbit antibodies against the rat Nedd4-1/2, Nedd4-2 and SGK1. Results All three proteins were extensively expressed in various regions of the rat cochlea. They were found in the stria vascularis, spiral ligament, organ of Corti, spiral limbus, spiral ganglion and Reissner's membrane. Conclusion Our findings suggest that there exists a Na+ transport system in the cochlea consisting of SGK1, Nedd4 isoforms and ENaC, which may work in concert to transport Na+ and to maintain homeostasis in the inner ear as they do in other tight epithelia.
