A Preliminary Study on the Differentiation of bone-marrow mesenchymal Stem Cells into Neuron-like Cells in Vitro after transfected by Brain- derived neurotrophic Factor Gene
- VernacularTitle:脑源性神经营养因子基因转染骨髓间充质干细胞体外诱导分化为神经元样细胞初步观察
- Author:
Qianxu LIU
;
Dinghua XIE
;
Guangui CHEN
- Publication Type:Journal Article
- Keywords:
Brain- derived neurotrophic factor gene;
Bone-marrow mesenchymalstem cells(BMSCs);
Differentiate;
Ineuron- like cells
- From:
Journal of Audiology and Speech Pathology
2009;17(3):252-255
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the function of bone-marrow mesenehymal stem eells(BMSCs) differ-entiating into neuron-like cells in vitro after transfected by human brain-derived neurotrophie factor (BDNF) gene. Methods Human BDNF genes were cloned and recombinant pcDNA3. 1(-)-BDNF plasmids were construc-ted. BMSCs from five guinea pigs were isolated and cultured while their morphologies were observed by microscope. The surface antigen was detected by flowcytometry. BDNF genes were transfected into BMSCs with electroporation, and the transfected BMSCs were induced by ratinoie acid(RA)after bolted by Geneticin-418 (G418), then the dif-ferentiated BMSCs were identified by immunocytochemistry. Results The culture ceils demonstroted the typical mor-phology and surface antigen of BMSCs. The transfected cells expressed neuron- specific enolase(NSE), Nestin and glial fi-brillary acid protein(GFAP) also secreted BDNF. Conclusion BMSCs transfected by BDNF genes can differentiate into neu-ron- like cells in vitro, electroporation can enhame the transfection efficiency, RA can promote cell induction.
