The Study of a New Approach to postauricular microinjection via the Round Window Membrane for Cochlear Gene transfection in Mouse
- VernacularTitle:耳后入路圆窗膜显微注射小鼠耳蜗基因转染新途径的研究
- Author:
Yanjun XU
;
Yinyan HU
;
Suoqiang ZHAI
;
Jianhe SUN
;
Jincao XU
;
Zhaohui HOU
;
Weidong SHEN
;
Ning YU
;
Shiming YANG
;
Dongyi HAN
- Publication Type:Journal Article
- Keywords:
Gene transfer;
Round window membrane;
Adenovirus;
Mouse;
Enhanced green fluores-cent protein(EGFP)
- From:
Journal of Audiology and Speech Pathology
2009;17(3):279-282
- CountryChina
- Language:Chinese
-
Abstract:
Objective To assess the feasibility of adenoviral vectors mediate cochlear gene transfer by postau-ricular microinjection through the round window membrane in mouse. Methods Twelve 5-week old C57BL/6J mice were selected for the study: 8 were implanted with Ad-EGFP by postauricular microinjection through the round window membrane, and 4 with artificial perilymphatic fluid. On postoperative days 5 and 14, the animals were sac-rificed and the surface preparation of cochleae was observed. Results Two animals died after operation. Bright green fluorescence in the cochleae was observed in Ad- EGFP groups. Gene expression on day 14 after operation was higher than that on day 5. However, the control group was free of fluorescence. Oonclusion The postauricular route of the cochlear gene transfer in mice is simple to operate with little side-effect. The technique of transgenic delivery into the inner ear through RWM by mieroinjection is feasible and effective.
