Genetic Polymorphism Analysis of Nine Short Tandem Repeat Loci in Han Population of Southern China
- VernacularTitle:中国南方汉族人群9个STR基因座多态性分析
- Author:
Dayue TONG
;
Hongyu SUN
;
Xinyao WU
;
Huiling LU
;
Jianjin LI
;
Lixian CHEN
- Publication Type:Journal Article
- Keywords:
short tandem repeat;
allelic frequency;
paternity testing;
individual-identification;
Han population
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2009;30(4):400-403
- CountryChina
- Language:Chinese
-
Abstract:
[Objective] To investigate the genetic polymorphism of nine short tandem repeat (STR) loci in Han population of Southern China.[Methods] The 9 STR loci (D11S2368,D12S391,D13S325,D18S1364,D22-GATA198B05,D6S1043,D2S1772,D7S3048,D8S1132) were amplified with STR_Typer_10_v1 kit for 1619 unrelated individuals of Han population in Southern China.The PCR products were analyzed with 3100 genetic analyzer and GeneMapper ID 3.1v software.The forensic efficiency parameters were calculated by PowerState V12.xls and the Hardy-Weinberg equilibrium was tested with Arlequin 3.11v software.[Results] The genetic polymorphism of 9 STR loci in Han population of Southern China was quite high.The heterozygosities (H) ranged from 0.818 to 0.879.The match probabilities (MP) ranged from 0.031 to 0.063.The powers of discrimination (PD) ranged from 0.937 to 0.970,the probabilities of exclusion (PE) ranged from 0.632 to 0.753,the polymorphism information contents (PIC) ranged from 0.80 to 0.88 and the typical paternity indices (TPI) ranged from 2.74-4.13,respectively.These data were in accord with Hardy-Weinberg equilibrium (P > 0.05).[Conclusion] Nine STR loci are highly polymorphic in Chinese Han population.They are new useful tools for paternity testing,individual identification,and for the research of human genetics and anthropology.