Analysis of Flavonoids in Semen Hoveniae by HPLC-ESI-MS
- VernacularTitle:枳棋子中黄酮类化合物的HPLC-ESI-MS分析
- Author:
Ling PENG
;
Kunping LI
;
Ying GAO
;
Weimin LI
- Publication Type:Journal Article
- Keywords:
Semen Hoveniae;
Flavone;
HPLC-ESI-MS
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
2009;20(4):341-343
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a HPLC-ESI-MS method for simultaneous analysis and determination of flavonoids in Semen Hoveniae. Methods The separation was performed on Acquity UPLCTM BEH C18 column (50 mm× 2. 1 mm,1.7 μm) with a mobile phase of formic acid aqueous solution (B) and MeOH (A). The flow rate was at 1.0 mL/min and detection wavelength was at 290 nm. Samples were analyzed in positive-ion detection model. Results Five kinds of flavonoids in Semen Hoveniae were separated well and were tentatively identified by ESI-MS and by reference to litera-ture data. Conclusion The method is accurate and rapid, and can be used for the identification of flavonoids in Semen Hoveniae.
