Determination of Baicalin and Berberine Hydrochloride in Compound Qinbo Granules by HPLC
- VernacularTitle:HPLC法测定复方芩柏颗粒中黄芩苷和盐酸小檗碱含量
- Author:
Ting ZHANG
;
Pinglan ZHOU
- Publication Type:Journal Article
- Keywords:
Compound Qinbo Granules;
Baicalin;
Berberinehydrochloride;
HPLC
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
2009;20(4):349-351
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a HPLC method for the determination of baicalin and berberine hydrochloride in Com-pound Qinbo Granules. Methods The gradient elution was performed on a Diamonsil C18 column using method-water (0. 05 % phosphate acid and 0. 1% trithylamine) as mobile phase, and the detection wavelength was 276 nm. Results The linear calibration curve of baicalin was obtained in the range of 10. 08～201.6 mg·L-1 with an average recovery of 98. 1% (RSD=1.11%). The linear calibration curve of berberine hydrochloride was in the range of 4. 08～81.6 mg·L-1 with an average re-covery of 97.9 % (RSD=1.24 %). Conclusion The method is simple and reproducible. It can be used for the quality con-trol of Compound Qinbo Granules.
