Determination of Gastrodin in Tianyuan Granula by HPLC
- VernacularTitle:HPLC法测定天元颗粒中天麻素的含量
- Author:
Ying FENG
;
Zhengyan XIA
;
Qiang XU
- Publication Type:Journal Article
- Keywords:
Tianyuan Granula;
Gastrodin;
HPLC
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
2009;20(4):354-356
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for the determination of gastrodin in Tianyuan Granula. Methods The sample was refluxed with methanol, then eluted with 80 % ethanol through the alumina column(neutral). The chromato-graphic conditions were as follows: Diamonsil Cts chromatographic column(250 mm ×4.6 mm, 5 μm) with a mobile phase of acetonitrile-0. 15 % phosphoric acid (1 : 99), the detection wavelength being at 220 nm and the flow rate be-good (r=0.9999 95, n=7).The average recovery was 99.09 % and RSD=1. 50 % (n=6). Conclusion The method for determination of gastrodin in Tianyuan Granula is accurate, and with a good reproducibility.
