Effects of Rifampicin on Rotenone-induced Oxidative Stress in Differentiated PC12 Cells
- VernacularTitle:利福平对鱼藤酮致分化PC12细胞氧化应激的影响
- Author:
Shiwen CHEN
;
Yuanlin SUN
;
Zhifen ZENG
;
Enxiang TAO
- Publication Type:Journal Article
- Keywords:
rifampicin;
rotenone;
reactive oxygen species;
reduced glutathione;
Parkinson--s disease
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2009;30(4):377-380
- CountryChina
- Language:Chinese
-
Abstract:
[Objective] To explore the effects of rifampicin on cell morphology,intracellular reactive oxygen species (ROS),reduced glutathione (GSH),and cell apoptosis in rotenone-induced differentiated pheochromocytoma (PC12) cells.[Methods] Rotenone was added in rat PC12 cells to develop a model of Parkinson's disease in vitro.Cell morphology was observed by microscope.Intracellular GSH was determined by a microplate reader.The intracellular ROS and the apoptosis rate were measured by flow cytometry.[Results] Compared with control group and rifampicin control group,GSH significantly decreased but ROS and apoptosis rate significantly increased in rotenone group.Compared with rotenone group,GSH significantly increased but ROS and apoptosis rate significantly decreased in a dose-dependent manner in rifampicin control group (100,200,and 300 μmol/L).[Conclusion] Rifampicin may reduce the damage of rotenone-induced differentiated PC12 cells through inhibiting oxidative injury in a dose-dependent manner.
