Polymorphism of Five X-STRs Loci with a New Pentaplex PCR
- VernacularTitle:用荧光复合扩增体系检测5个X染色体短串联重复序列基因座的多态性
- Author:
Qiuling LIU
;
Dejian LV
;
Hu ZHAO
;
Xinguo LI
;
Huling LU
;
Hongyu SUN
;
Yanfang LIANG
;
Xinyao WU
- Publication Type:Journal Article
- Keywords:
X-STR;
multiplex PCR;
polymorphism;
Guangdong Han population in China
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2009;30(4):404-407
- CountryChina
- Language:Chinese
-
Abstract:
[Objective] To learn about the genetic diversity,we studied the five X-chromosomal STR (X-STR) loci in Guangdong Han Nationality Groups.[Methods] The five Loci (DXS6803,DXS981,DXS6809,DXS6789,and DXS7132) were amplified in a pentaplex PCR reaction.PCR products were analyzed using capillary electrophoresis and ABI prism 3100 Genetic Analyzer,with GeneMapper ID 3.1 Analysis Software.[Results] A total of 363 individuals (181 unrelated male and 182 unrelated female) from Guangdong Han population were tested,54 alleles were observed for these loci.Polymorphism information content is 0.6935 ～ 0.8177.Power of discrimination in females was 0.8976 ～ 0.9562.Mean exclusion chance for X-STR in standard trios with daughters was 0.7805 ～ 0.8467.[Conclusion] The five loci in the multiplex system provide high polymorphism information for forensic identification and paternity testing,particularly for difficult paternity deficiency cases.
