Effects of Honokiol on Proliferation and Apoptosis on U937 Cells
- VernacularTitle:和厚朴酚对人白血病细胞系U937细胞增殖和凋亡的影响
- Author:
Fang XUE
;
Zhiyong CHENG
;
Lin YANG
;
Shihui LI
;
Jingyu ZHANG
;
Li YAO
;
Ling PAN
- Publication Type:Journal Article
- Keywords:
Honokiol;
U937 cell line;
membrane potential,mitochondrial;
Caspase3/7
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2009;30(4):408-412
- CountryChina
- Language:Chinese
-
Abstract:
[Objective] To investigate the anti-proliferative and apoptosis effect induced by Honokiol (HNK) on human myeloid leukemia cell line U937 cells in vitro.[Methods] After treated with different concentration of HNK,Hoechst33342 fluorescent staining was used to detect cell apoptosis;the growth inhibition ration of U937 cells and PBMCs were analyzed by MTT assay;the apoptosis ration was detected by flow cytometry;mitochondrial membrane potential was explored by rhodamine 123 stain;Caspase3/7 protein activity kit was used to test the Caspase3/7 activity;the Caspase-3 and Caspase-7 mRNA levels were detected by real-time fluorescent relative-quantification reverse transcriptional PCR (FQ-PCR).[Results] Honokiol could significantly inhibit the proliferation of U937 cells in terms of the indexes of IC50/U937 11.8 μg/mL and IC50/PBMCs 40.3 μg/mL,and the anti-proliferative effect was in a time and concentration dependent manner;Flow cytometry analysis manifested that Honokiol could induce U937cells apoptosis by Annexin V/PI double Annexin V/PI fluorescein stain;Honokiol significantly inhibited the mitochondrial membrane potential of U937 cells and enhanced the ability of Caspase3/7 and the mRNA expression levels,but not the PBMCs.[Conclusion] HNK can inhibit U937 cells proliferation and induce cells apoptosis via activating Caspase 3/7.
