Expression of MICA mRNA and Protein in Osteosarcoma
- VernacularTitle:骨肉瘤组织中MICA基因mRNA和蛋白的表达
- Author:
Shanming LU
;
Ling XUE
;
Ping XIAO
;
Yang LI
;
Qinghua CAO
;
Hui QIAO
- Publication Type:Journal Article
- Keywords:
osteosarcoma;
MHC class I chain-related A;
MICA;
expression
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2009;30(4):441-445
- CountryChina
- Language:Chinese
-
Abstract:
[Objective] To investigate the expression of MICA mRNA and protein in osteosarcoma and give a more comprehensive understanding to its immune evasion.[Methods] RT-PCR was used to analyze MICA mRNA in 11 osteosarcoma tissues and 5 osteosarcoma cell lines.MICA expression was examined in 66 paraffin-embedded osteosarcoma tissues and 6 paraffin-embedded normal bone tissues by immunohistochemistry,and MICA protein in 9 fresh osteosarcoma tissues was detected by Western blot too.MICA surface expression was estimated by flow cytometry.[Results] Nine of eleven (81.8%) osteosarcoma specimens and all of the five cell lines consistently expressed MICA mRNA.Up-regulation of MICA expression was found in osteosarcoma (34/66,51.6%),compared with normal bone tissues (0/6,0%).The cell lines Saos-2,MG63,and HOS showed positive surface MICA expression,while the U2OS and OS732 showed very limited expression.[Conclusion] MICA mRNA and protein predominantly expressed on osteosarcoma tissues and cell lines.
