Expression of Wip1 mRNA in Non-small Cell Lung Cancer by Real-time PCR
- VernacularTitle:实时荧光定量 PCR检测野生型p53介导蛋白磷酸酶mRNA在非小细胞肺癌中的表达
- Author:
Wei ZHANG
;
Jixing ZHAO
;
Honghe LUO
;
Jianjun LU
;
Jun MA
;
Yong GU
- Publication Type:Journal Article
- Keywords:
non-small cell lung cancer;
Wip1;
real time fluoresene quantitative PCR
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2009;30(4):463-467
- CountryChina
- Language:Chinese
-
Abstract:
[Objective] The aim of this study was to establish a quantitative SYBR Green Ⅰ real-time PCR method for detection of wide-type p53-induced phosphatase 1 (Wip1 or PPM1D) gene expression level in non-small cell lung cancer (NSCLC),and to investigate the relationship between Wip1 mRNA expression level and the clinicopathological characters.[Method] Real-time PCR was employed to determine the expression level of Wip1 mRNA in 44 specimens of NSCLC tissues and their adjacent normal tissues.[Results] In the 44 specimens,the expression of Wip1 mRNA in both cancer tissues and adjacent normal lung tissues were positive.Wip1 gene was overexpressed in 17 specimens among 44 NSCLC specimens.The rate was 38.6%.The relative level of Wip1 mRNA in NSCLC tissues was significantly higher than the adjacent normal lung tissues (Ratio ＝ 2.1644 ± 1.394,P ＜ 0.01).The expression of Wip1 mRNA was also correlated with pathological staging (F ＝ 5.08,P ＝ 0.013).[Conclusion] The established SYBR Green Ⅰ quantitative real-time PCR method can successfully detect the expression level of Wip1 mRNA.The results suggested that Wip1 may be involved in the development of NSCLC.
