Comparing Results with Immature Germinal Vesicle Oocytes From Different In-vitro Fertilization Cycles
- VernacularTitle:不同体外受精周期所获生殖泡期卵母细胞体外成熟后结果比较
- Author:
Cong FANG
;
Benyu MIAO
;
Yiping ZHONG
;
Canquan ZHOU
;
Guanglun ZHUANG
- Publication Type:Journal Article
- Keywords:
germinal vesicle;
intracytoplasmic sperm injection;
in vitro maturation
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2009;30(4):473-476
- CountryChina
- Language:Chinese
-
Abstract:
[Objective] This study compared outcomes of in vitro maturation (IVM) and in vitro fertilization (IVF) intracytoplasmic sperm injection (ICSI) cycles after IVM of immature germinal vesicle (GV) oocytes.[Methods] ICSI was performed on metaphase II (MII) oocytes retrieved in 163 IVF-ICSI cycles (group I;n ＝ 987) or matured from GV stage oocytes in IVF-ICSI ( group II;n ＝ 132) and 37 IVM cycles ( group III;n ＝ 235).Fertilization and cleavage rates and embryo quality were compared among the three groups.[Results] The fertilization rate,cleavage rate and top quality embryos rate were higher in group I than group II and group III (84.9%,98.1%,and 61.6%;72.0%,90.5% and 22.1%l;75.3%,94.4%,and 25.1%,respectively).Blastomere numbers and morphology scores were highest in group I (P ＜ 0.05),but no significant differences existed between group II and group III.[Conclusion] The morphology of embryos developed from in vivo MII oocytes was superior to those from in vitro matured MII oocytes.No significant difference was observed in embryo morphology from immature GV oocytes in IVF and IVM cycles.
