Effect of Extract of Chrysanthemum Indicum on Tumor Necrosis Factor-α Expression and Neutrophil Function in Chronic Bronchitis Rats
- VernacularTitle:野菊花提取物对慢性支气管炎大鼠TNF-α及中性粒细胞功能的影响
- Author:
Yun SU
;
Yong HUANG
;
Juan LI
;
Shaobo SUN
;
Yi ZHAN
;
Chunlu YAN
;
Yongqi LIU
- Publication Type:Journal Article
- Keywords:
Chrysanthemum indicum;
Chronic bronchitis;
Tumor necrosis factor-α;
Neutrophil;
Phagocytosis;
Respiratory burst
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
2009;20(4):300-303
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of the extract of Chrysanthemum Indicum(CI)on the expression of tumor necro-sis factor-α (TNF-α) and neutrophil function in chronic bronchitis (CB)rats and to explore the therapeutic mechanism for chronic bronchitis. Methods The extract of CI was prepared. Wistar rats were randomly divided into blank control group, model group, Chuanxiling group, and low-, middle-and high-dose CI extract groups. Rat model of CB was established by intratracheal injection with low-dose lipopolysaccharide. After drug intervention, the expression of TNF-α in rat serum and bronchoalveolar lavage fluid was detected by ELISA method. Phagocytic function of the neu-trophil and respiratory burst of the rats were measured by flow cytometry (FCM). Results Compared with the blank con-trol group, the phagocytic function of the neutrophil, respiratory burst of the rats, and the expression of TNF-α in rat serum and bronchoalveolar lavage fluid of the model group were increased significantly. CI extract significantly decreased the abnormal rising of the above indexes. Conclusion Down-Regulation of TNF-α expression, and decrease of the neutrophil phagocytic function and rat respiratory burst may be one of the therapeutic mechanisms of Chrysanthemum In-dicum extract for the treatment of CB.
