Simultaneous Determination of Campanulin, Paeoniflorin and Hydroxysafflor Yellow A in Buyang Huanwu Decoction by HPLC
- VernacularTitle:HPLC法同时测定补阳还五汤中毛蕊异黄酮苷、芍药苷、羟基红花黄色素A的含量
- Author:
Jun ZHANG
;
Tingbe CHEN
;
Jun CHEN
;
Daimei WANG
;
Gang WEI
- Publication Type:Journal Article
- Keywords:
Buyang Huanwu Decoction;
Campanulin;
Paeoniflorin;
Hydroxysafflor yellow A;
Determination;
HPLC
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
2009;20(4):373-376
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a HPLC method for the simultaneous determination of campanulin, paeoniflorin and hydrox-ysafflor yellow A in Buyang Huanwu Decoction. Methods A Kromasil C18(4. 6 mm × 250 mm, 5 μm) column was adopted. The mobile phase consisited of methanol and acetonitrile(26 : 2) (A), and 0. 7 % phosphoric acid solution(B), in gradient e-lution (0～10 min, 25 % →35 % A; 10～25 min, 35 %→40 % A). The flow rate was at 1.0 mL·min-1. The column tem-perature was 35 ℃;, and the detection wavelengths were set at 260nm for campanulin, 230 nm for paeoniflorin and 403 nm for hydroxysamor yellow A. Results The linear ranges of campanuliu, paconiflorin and hydroxysafflor yellow A were 0. 0539～1. 078 μg (r=0. 999 5), O. 4160～8. 320μg (r=0. 999 8), and 0. 0418～0. 8352 μg (r=0. 999 5) respectively; the av-erage recovery was 99. 4 % (RSD=1.5 %), 100. 6 % (RSD=1.7 %), and 101.0 % (RSD=1.9 %) respectively. Conclu-sion The method is simple, feasible and reproducible, and can be used for the quality control of Buyang Huanwu Decoction.
