Isolation,identification and sequence analysis of the strain JL/07/SW with deletion mutations of PRRSV
- VernacularTitle:PRRSV缺失变异毒株JL/07/SW株的分离鉴定及序列分析
- Author:
Zhijie LI
;
Zhuang DING
;
Keyin MENG
;
Hua XUAN
;
Guiping WANG
;
Enpeng GAO
;
Yanlong CONG
;
Lianzhi MU
- Publication Type:Journal Article
- Keywords:
PRRSV;
isolation;
identification;
sequence analysis
- From:
Chinese Journal of Veterinary Science
2009;29(7):830-835
- CountryChina
- Language:Chinese
-
Abstract:
One strain of PRRSV was isolated from tissue of piglets died of obvious respiratory syndrome and high fever.It was identified as a strain of American type PRRSV by serological test and gene sequencing.According to American type PRRSV VR-2332,we designed 3 pairs of primers to aim directly at N gene,GP5 gene and variation sequence of NSP2.The results of sequence analysis indicated a discontinuous deletion of 30 amino acids in nonstructural protein 2 (NSP2).N and GP5 gene is conservative relatively.
