Cytokine Response of the Synovium-like Membrane to the Particulate Biomaterial in Murine Air-Pouch Model.
- Author:
Kang Sup YOON
1
;
Seung Baik KANG
Author Information
1. Department of Orthopaedic Surgery, Seoul Municipal Boramae Hospital, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Biomaterial;
Particle;
Macrophage;
T-cell;
Cytokine
- MeSH:
Alloys;
Animals;
Arthroplasty, Replacement;
Biocompatible Materials;
Cytokines;
Cytosine;
Humans;
Interleukin-4;
Interleukin-6;
Joints;
Macrophages;
Membranes*;
Mice;
Osteolysis;
RNA, Messenger;
T-Lymphocytes;
Titanium;
Tumor Necrosis Factor-alpha
- From:Journal of Korean Orthopaedic Research Society
1999;2(1):11-17
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVES: The purpose of this study was to evaluate and classify the synovium-like membrane as either inflammatory (foreign body-like) or immune (T-cells present), generated during the development of aseptic loosening in the patients with joint replacement arthroplasties. METHODS: We used the murine air-pouch model, developed in BALB/c mouse, to make artificial synovial cavity and assessed the cytokine responses of the synovium-like membrane to the particulate biomaterials such at pure titanium, Ti6Al4V alloy, CoCr alloy and UHMWPE. Each biomaterial injected into air-pouch of 20 mice respectively. The mRNAs of various cytokines (IL-1 beta, IL-6, TNFalpha, IL-4, gammaIFN) and TCR Calpha chain were extracted from the synovium-like membranes and RT-PCR reactions were carried out using appropriate primers. RESULTS: IL-1beta, IL-6 and TNFalpha showed an increased response to the all kinds of particulate biomaterials. IL-4, gammaIFN and TCR Calpha chain did not show any distinct responses compared to control group and various kinds of biomaterials failed to show any, significant differences from each other in cytosine responses. CONCLUSION: T-cell or immune cytokine responses had not been found in this particle reaction and it seemed to be that the osteolysis in aseptic loosening would be a biologic reaction to the particulate biomaterials due to an inflammatory process.
