Expressing of bovine viral diarrhea-mucosal disease virus Changchun184 strain of E2 gene in BCG
- VernacularTitle:牛病毒性腹泻-黏膜病病毒Changchun184株E2基因在卡介苗中的表达
- Author:
Rui DU
;
Yan DIAO
;
Junyou HAN
;
Xichen ZHANG
;
Kun SHI
- Publication Type:Journal Article
- Keywords:
bovine viral diarrhea virus (BVDV);
E2 gene;
recombinate BCG
- From:
Chinese Journal of Veterinary Science
2009;29(7):854-857
- CountryChina
- Language:Chinese
-
Abstract:
E2 gene of BVDV Changchun 184 strain was cloned and inserted into the shuttle expression plasmid vector pMV261,the recombinant shuttle plasmid pMV261-E2 was constructed.Then pMV261-E2 was transformed into BCG successfully and obtained recombinant BCG which was resistive to kanamyein.The recombinant BCG were identifieated by PCR.E2 gene expression in recombinant BCG was induced in 45℃,then the SDS-PAGE and western blotting was used to analyze the expression product.The results indicated the BVDV E2 gene was expressd in BCG successfully.
