Effects of fluoride on the expression of functional genes related with the metabolism of thyroid hormone in FRTL cells
- VernacularTitle:氟化物对FRTL细胞甲状腺激素代谢相关基因表达的影响
- Author:
Peng JIANG
;
Weidong ZHANG
;
Chunyan CHAI
;
Rui XIAO
;
Mingxing DING
;
Guoyan LIU
- Publication Type:Journal Article
- Keywords:
fluoride;
FRTL cells;
thyroglobulin (TG);
thyroid peroxidase (TPO);
sodium iodide symporter(NIS)
- From:
Chinese Journal of Veterinary Science
2009;29(7):885-888
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the effects of fluoride on the expression of thyroglobulin (TG),thyroid peroxidase(TPO),odium iodide symporter (NIS) genes in FRTL cells,FRTL cells cultured in vitro were treated in logarithmic phase with sodium fluoride at different concentration of 20.0,10.0,5.0,2.5,1.25 mg/L,respectively.After 72 h,the cells were collected and semi-quantitative RT-PCR for TG mRNA,TPO mRNA and NIS mRNA and β-actin was performed.The results showed that compared with the control cells,the expression levels of TG gene in FRTL cells treated with lower concentration of sodium fluoride increased compensatively,but decreased significantly (P<0.05) with higher concentration(>5.0 mg/L);the RT-PCR products of TPO and NIS in FRTL cells treated with all concentration of sodium fluoride reduced,some of them were significant (P<0.05).It is concluded that fluoride can reduce the expression of TG,TPO,NIS genes in thyroid cells,conseguently cause dysfunction" of thyroid in uptaking and unilizing of iodine,and synthesizing,storing,secreting of thyroid hormones.
