Influencing factors in the refolding process of artificial molecular chaperone assisting chicken IL-18 recombination protein
- VernacularTitle:人工分子伴侣系统辅助鸡IL-18重组蛋白复性过程中研究的影响因素
- Author:
Xinhua WANG
;
Jingdong HU
;
Na KONG
;
Hongmei LI
;
Hongkun ZHAO
- Publication Type:Journal Article
- Keywords:
artificial molecular chaperone;
chicken IL-18 recombination protein;
refolding;
refolding yield
- From:
Chinese Journal of Veterinary Science
2009;29(7):905-908
- CountryChina
- Language:Chinese
-
Abstract:
The recombinant plasmid of mChlL-18 prokaryotic expression was transformed into E.coli BL21(DE3) strain and then induced by IPTG at 37℃.After crushed and washed,the expressing inclusion bodies were thoroughly denatured with 6 mol/L guanidine hydrochloride.Then according to experiment design,the effects of rChlL-18 protein refolding yield at different densities were investigated by the systems of artificial chapercne at different densities.Experiment results indicate that there is a optimal condition on assiting rChIL-18 protein by using the artificial chaperone technique.The optimal condition can improve the refolding yield of rChIL-18 protein,and then the expressed product of fusion chicken IL-18 gene in E.coli has a relativity high bioactivity.
