Effects of recombinded resistin on pyruvate carboxylase mRNA expression and activity in culture bovine hepatocyte in vitro
- VernacularTitle:重组抵抗素对犊牛肝细胞PC mRNA丰度及其活性的影响
- Author:
Aodi CHEN
;
Pengfei HE
;
Guowen LIU
;
Chengzhi CHEN
;
Zhe WANG
- Publication Type:Journal Article
- Keywords:
recombinded resistin;
hepatocyte;
pyruvate carboxylase;
calf
- From:
Chinese Journal of Veterinary Science
2009;29(7):924-927
- CountryChina
- Language:Chinese
-
Abstract:
A single factor duplicate test was designed to investigate whether bovine recombinded resistin impacts the expression of pyruvate carboxylase (PC) mRNA and the activity of PC in vitro culture bovine hepatocyte.Bovine recombinded resistin was added to the media with 0,25,50,100,200 and 400 ng/L.Abundance of PC mRNA in bovine hepatocyte,which was cultured with bovine recombinded resistin for 12 hours,was determined by real-time fluorescence quantitative RT-PCR,and activity of PC was determined by colourimetry.The results showed that bovine recombinded resistin could downregulate the expression of PC mRNA and the activity of PC in vitro culture bovine hepatocyte.
