Delayed effect of isoflurane on hippocampal proteome after anesthesia in adult and aged rats
- VernacularTitle:异氟烷对成龄和老龄大鼠海马蛋白质组延迟相的差异性影响
- Author:
Kaiming DUAN
;
Wen OUYANG
;
Manhong CHEN
;
Yuefeng XIA
;
Saiying WANG
- Publication Type:Journal Article
- Keywords:
isoflurane;
hippocampus;
delayed;
proteome;
aged;
rat
- From:
Journal of Central South University(Medical Sciences)
2009;34(7):589-594
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the delayed alteration of hippocampus proteome after an-esthesia with isoflurane in aduh and aged rats. Methods Ten 8-month-old SD rats were randomly divided into group Caduh and group Iadult (5 in each group) , and another ten 22-month-old SD rats were randomly divided into group Caged and group Iaged (5 in each group). The rats in group Iadult and group Iaged received 2 h anesthesia with 1.2 % isoflurane. The rats in group Cadult and group Caged inhaled 40% oxygen for contrast. The hippocampal proteome of each rat was measured by 2-dimensional gel electrophoresis and mass spectrometry. Results The vital signs of the rats in group Iadult and group Iaged were stable. There were 878±34 protein spots in group Cadult, 864±49 protein spots in group Iadult, 834±47 in group Caged, and 819±24 in group Iaged. There were 12 (4/8)different protein spots between group Iadult and group Cadult. There were 11 (3/8) different protein spots between group Iaged and group Caged. All of the protein spots were identified by MALDI-TOF-MS. Most of the different proteins were related to metabolism, anti-oxidation, and signal conditioning of synapse. Conclusion Isoflurane may cause the alteration of hippocampal pro-teome in rats, which is age-related.
