Effect of atorvastatin on inflammatory infiltration in the lung of rabbits with hypercholesterolemia
- VernacularTitle:阿托伐他汀对高胆固醇血症兔肺内炎性浸润的影响
- Author:
Shangjie WU
;
Guiyuan LI
;
Jing ZHANG
;
Yan ZHANG
;
Yan GAN
;
Xiqian XING
;
Ping CHEN
;
Shuiping ZHAO
- Publication Type:Journal Article
- Keywords:
atorvastatin;
hypercholesterolemia;
inflammation;
NF-κB;
IL-6;
cell proliferation
- From:
Journal of Central South University(Medical Sciences)
2009;34(7):608-615
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the effect of atorvastatin on the hypercholesterolemia in-duced lesion in the lung. Methods Fifteen male New Zealand rabbits were randomly assigned into a control group (n=5) , a high-cholesterol forage group (n=5) , and an atrovastatin treatment group (n=5). The control group received normal forage, but the high-cholesterol group and atrovastatin treatment group received high-cholesterol forage. From the 9 th week, the atrovastatin treatment group was added atorvastatin, and the experiment stopped at the end of the 14th week. At the beginning of the experiment and at the 8 th, 14 th week, blood cholesterol and body weight were detected. At the 14th week, bronchial alveolar lavage (BAL) was performed in vitro after the rabbits were executed; pathological examinations were determined in the lung tissues by staining with hamatoxylin-eosin. Oil red 0 and the activities of NF-κB in the alveolar macrophages (AMs) were investigated by immuno-cytochemistry. Proliferative cell nuclear antigen in the lung tissues was adopted by immunohistochem-istry, and the concentrations of IL-6 in the serum, BALF and the culture supematants of AMs were measured by ELISA. Pulmonary tissue paraffin section was stained with hamatoxylin-eosin. Results Atorvastatin reduced inflammatory infiltration, AM NF-κB activation, and cell proliferation in the lung, but raised IL-6 level. Conclusion Hypercholesterolemia-induced pulmonary inflammation is attenuated by atorvastatin.
