Two-dimentional liquid phase chromatographic proteome profiles in Uighur women with uterine cervix cancer
- VernacularTitle:维吾尔族妇女宫颈癌患者血清蛋白质组的二维液相色谱分析
- Author:
Xia GUO
;
Abudula ABULIZI
;
Guizhen WU
;
Kaijiang LIU
- Publication Type:Journal Article
- Keywords:
serum proteome;
2-D liquid phase chromatography;
cervical carcinoma;
Uighur women
- From:
Journal of Central South University(Medical Sciences)
2009;34(7):624-629
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish differential protein expression profile specific for serum proteome of cervical carcinoma in Uighur women by protein 2-dimensional liquid phase chromatogra-phy. Methods We collected and prepared sera of cervical carcinoma from Uighur women, and sepa-rated and processed data using 2-dimensional liquid phase chromatography system specific for protein (ProteomeLabTM PF-2D ). Results A differential expression profile of serum proteome based on characteristics of protein isoelectrie point and hydrophobic features was successfully established. Fifty-six differentially expressed protein spots were found, among which there was an obvious difference in 12 peaks. Conclusion Two-dimensional liquid phase chromatography is an effective and feasible method to establish differential expression profile of serum proteome and to offer a new way for the screening of serum protein markers of Uighur women with cervical carcinoma.
