Effect of triptolide on the proliferation and apoptosis of human epidermal squamous cell carcinoma cell line A431 in vitro
- VernacularTitle:雷公藤内酯醇对皮肤鳞状细胞癌A431细胞株增殖与凋亡的影响
- Author:
Mingliang CHEN
;
Shuai TAN
;
Guiying ZHANG
;
Mei YI
;
Dan JIAN
;
Hongfu XIE
;
Xiang CHEN
- Publication Type:Journal Article
- Keywords:
triptolide;
carcinoma,squamous cell;
proliferation;
cell cycle;
apoptosis
- From:
Journal of Central South University(Medical Sciences)
2009;34(7):638-641
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of triptolide on the proliferation and apoptosis of human epidermal squamous cell carcinoma cell line A431 in vitro. Methods Human epidermal squamous cell carcinoma cell line A431 was cultured. After the treatment with triptolide, the inhibi-tion of cellular growth was determined by measuring MTT dye absorption of the living cells. Light mi-croscope showed morphological changes. The cell cycle and apoptosis rate were assessed by flow cy-tometry. Results Triptolide could significantly inhibit the proliferation of A431 cells in a dose- and time-dependent manner. Triptolide could also cause cell morphological changes ( the number of float-ing cells and nuclear pyknosis increase), induce cell apoptosis, and change the distribution of cell cycle phase in A431 cells. Compared with the control group, the G0/G1 phase A431 cell rate in-creased and the rate of S phase cell decreased in TP-treated group. Cell cycles were obviously inhibi-ted by triptolide in G0/G1 phase (both P<0.05). Conclusion TP could play an anti-tumor role by effectively inducing cell apoptosis and inhibiting the proliferation of A431 cells.
