Technetium [99Tc] methylenediphosphonate inhibits osteoclast formation from PBMCs in patients with rheumatoid arthritis
- VernacularTitle:锝[99Tc]亚甲基二膦酸盐抑制类风湿关节炎患者外周血单核细胞向破骨样细胞转分化
- Author:
Ying JI
;
Xiaocong HUO
;
Hao ZHANG
- Publication Type:Journal Article
- Keywords:
osteoclast;
rheumatoid arthritis;
tecgnetium[99Tc]methylenediphosphonate
- From:
Journal of Central South University(Medical Sciences)
2009;34(7):684-688
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the influence of technetium [99Tc] methylenedipho-honate (99Tc-MDP) on osteoclastogenesis induced by receptor activator of NF-κB ligand (RANKL) and macrophage-colony stimulating factor (M-CSF)in peripheral blood mononuclear cells in patients with rheumatoid arthritis, and to study the mechanism of 99Tc-MDP in osteoclast differentiation. Methods The monocytes/macrophages were isolated from peripheral blood in patients with rheumatoid arthri-tis, incubated in RPMI-1640 with receptor activator of NF-κB ligand (RANKL, 25 μg/L), macro-phage-colony stimulating factor (M-CSF, 25μg/L ) and different concentrations of 99Tc-MDP (5, 10, 20,and 50 mg/L) for 4,12, and 20 days. Tartrate resistant acid phosphatase staining was used to observe the formation of osteoclasts. Results After 12 or 16 days culture of peripheral blood mononuclear cells, plenty of large nultinuclear cells could be found on the coverslips. 99Tc-MDP markedly inhibited those changes and the inhibitory effects were increased as the concentration of 99Tc-MDP increased (P<0.05). Conclusion 99Tc-MDP probably has some protective effect on rheumatoid arthritis by inhibiting osteoclast formation.
