Regulation of the Expression of NEF3 in RA Induced Differentiation of SH-SYSY Neuroblastoma Cells
- VernacularTitle:RA诱导SH-SY5Y细胞分化中NEF3基因表达的调控
- Author:
Li ZHANG
;
Jinhua SHEN
;
Hui DAI
;
Ye ZHANG
;
Yufei SHEN
- Publication Type:Journal Article
- Keywords:
NEF3;
RA;
Brg1;
Chromatin remodeling
- From:
Journal of Medical Research
2009;38(8):18-22
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the regulation of NEF3 expression in SH - SY5Y after RA induction. Methods The expression of NEF3 and Brg1 genes was individually detected by realtime RT - PCR and Western blot analyses. Then, the CAT reporter driven by 5' flanking region ofNEF3 gene (pBLCAT3 -2. 8k - NEF3) was co - transfccted with eukaryotic expression plasmids of pBJ5 - Brgl or pBJ5 - Brg1 - NTP into SH - SY5Y respectively. Promoter activity of the NEF3 gene was detected by competitive RT - PCR assay. Results Similar changes in the mRNA expression of NEF3 and Brg1 and that of the protein expression of Brgl were detected in Sh - SY5Y cells af-ter RA treatment for 12 -24h. Wild type Brgl can promote the expression of pBLCAT3 -2.8k -NEF3 promoter after RA induction, but not mutant Brg1. Conclusion Brgl helpes regulating the expression of NEF3 gene possibly via changes in chromatin conformation in the SH -SY5Y cells after RA induction.
