On-column Enrichment with Field Amplified Sample Stacking in Capillary Zone Electrophoresis
- VernacularTitle:毛细管区带电泳中场增强进样柱内富集的非线性特征
- Author:
Feng CAO
;
Weibing ZHANG
;
Yukui ZHANG
- Publication Type:Journal Article
- Keywords:
Capillary zone electrophoresis;
enrichment;
sample injection
- From:
Chinese Journal of Analytical Chemistry
2009;37(7):965-969
- CountryChina
- Language:Chinese
-
Abstract:
FASS(field amplified sample stacking) is a technique for on-column enrichment in capillary zone electrophoresis. The distribution of solute in column during injection can be divided into two parts: the stack zone in running buffer and the sample solution zone imported by electroosmosis. Based on the investigation of transfer of solute in column, the results showed that the relationship between the length of two zone and injection time was nonlinear, so the relationship of injecting size that related with the mobility of solute and injection time. The increasing of the enrichment effect will not synchronize with the increasing of injection size, and the enrichment effect is decreased due to the laminar flow. To hold a basic constant column efficiency and desire enrichment effect, the ratio of mobility of solute in two zones should be maximum, and the injection time should also match with the ratio.
