Detection of Human Chorionic Gonadotropin by Highly Sensitive Magnetic EnzymE-linked Chemiluminescent Immunoassay
- VernacularTitle:高灵敏度化学发光磁酶免疫法检测人绒毛膜促性腺激素
- Author:
Juntao LIU
;
Ruping LIU
;
Mixia WANG
;
Chunxiu LIU
;
Jinping LUO
;
Xinxia CAI
- Publication Type:Journal Article
- Keywords:
Chemiluminescence;
magnetic immunoassay;
human chorionic gonadotropin
- From:
Chinese Journal of Analytical Chemistry
2009;37(7):985-988
- CountryChina
- Language:Chinese
-
Abstract:
A highly sensitive magnetic enzymE-linked chemiluminescent immunoassay method was developed for the detection of human chorionic gonadotropin(HCG). The monoclonal antibody was covalently coupled on the surface of carboxylated magnetic beads to generate magnetic-biotargeting; Alkaline phosphatase(ALP) was utilized as a labeled reagent of another monoclonal antibody, whereas 3-(2-spimadamantane) 4-methoxy-4-(3-phosphoryloxy)phenyl-1,2-dioxetane(AMPPD) was utilized as the chemiluminescent substrate. Based on this concept, a highly sensitive chemiluminescent immunoassay method was established to test HCG. Then, several modifications were made to optimize the method, and the detection sensitivity and procedure were improved accordingly. The detection of the assay could be fulfilled within 60 min and the test result of HCG concentration was linear over the range of 0.15 150 IU/L with good relativity(r=0.960). The relative standard deviation(RSD) were below 5% and the sensitivity of this method was 0.15 IU/L. The proposed method with wide linear range, simple operation and fast detection showed good prospect in practical application on-site.
