Characterization of Recombinant Human Necrosis Factor-α ReceptorⅡ:IgG Fc Fusion Protein by Nano Ultra-high Performance Liquid Chromatography-Electrospray Ionization Mass Spectrometry Tandem Mass Spectrometry
- VernacularTitle:超高效纳升液相色谱-电喷雾串联质谱鉴定重组人Ⅱ型肿瘤坏死因子受体-抗体融合蛋白
- Author:
Zhaofang BAI
;
Birong LIN
;
Ping LI
;
Weihua LI
;
Bingyu LIU
;
Hongxia WANG
- Publication Type:Journal Article
- Keywords:
Recombinant human necrosis factor-α receptorⅡ∶ IgG Fc fusion protein;
nano ultra-high perfor-mance liquid chromatography-electrospray ionization mass spectrometry tandem mass spectrometry;
protein identification
- From:
Chinese Journal of Analytical Chemistry
2009;37(7):1025-1028
- CountryChina
- Language:Chinese
-
Abstract:
The Nano ultra-high performance liquid chromatography-electrospray ionization mass spectrometry tandem mass spectrometry(UPLC-ESI-MS/MS) was used to characterize the primary protein. Samples were digested by trypsin, followed by liquid chromatography-tandem mass spectrometry analysis and database search. Five peptides matched with tumor necrosis factor receptor and seven peptides matched with human IgG1 fragment of the Fc. Further analysis demonstrated that seven peptides all matched with the IgG1 Fc but only partly peptides matched with the other subtypes. RhTNFR:Fc is fused by IgG1 Fc but not other subtypes.
